Multivariate Statistical Tools for the Evaluation of Proteomic 2D-maps: Recent Achievements and Applications
نویسندگان
چکیده
Two dimensional polyacrylamide gel electrophoresis (2D-PAGE) maps represent an unavoidable tool in many fields connected with proteome research, such as development of new diagnostic assays or new drugs. Unfortunately the information contained in the maps is often so complex that its recognition and extraction usually requires complex statistical treatments. Statistics accompanies many phases of 2D-PAGE maps management from the spot revelation to maps matching, as well as the extraction and rationalisation of useful information. This review describes and reports the most recent achievements in the field of statistical tools applied to proteome research by two-dimensional gel electrophoresis (2D-GE). The first section is devoted to briefly describe the theoretical aspects of the multivariate methods mostly adopted in this field such as Principal Component Analysis, Cluster Analysis, Classification methods, Artificial Neural Networks. The most recent applications are then described explaining the analysis of spot volume datasets from standard differential analysis as well as the direct analysis of 2D maps images. Applications are also reported about the use of multivariate tools in the analysis of DNA and RNA profiles.
منابع مشابه
Mathematical Tools and Statistical Techniques for Proteomic Data Mining
Proteomics is the study of and the search for information about proteins. The development of mass spectrometry (MS) such as matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) MS and imaging mass spectrometry (IMS), greatly speeds up proteomics studies. At the same time, the MS and IMS applications in medical science give rise to many challenges in mathematics and statistic...
متن کاملEconomic Statistical Design of Multivariate T^2 Control Chart with Variable Sample Sizes
Today, quality improvement and cost reduction are key factors for achieving business success, growth and position. One of the primary tools for quality improvement and cost reduction in online activities of statistical process control is control charts. As the need for monitoring several correlated quality characteristics is extensively growing, the use of multivariate control charts become...
متن کاملRecent analytical applications of magnetic nanoparticles
Analytical chemistry has experienced, as well as other areas of science, a big change due to the needs and opportunities provided by analytical nanoscience and nanotechnology. Now, nanotechnology is increasingly proving to be a powerful ally of analytical chemistry to achieve its objectives, and to simplify analytical processes. Moreover, the information needs arising from the growing nanotechn...
متن کاملComparing Bivariate and Multivariate Methods in Landslide Sustainability Mapping: A Case Study of Chelchay Watershed
1- INTRODUCTION In the last decades, due to human interventions and the effect of natural factors, the occurrence of landslide increased especially in the north of Iran, where the amount of rainfall is suitable for the landslide occurrence. In order to manage and mitigate the damages caused by landslide, the potential landslide-prone areas should be identified. In landslide susceptibili...
متن کاملSimulation of Smoke Emission from Fires in High-Rise Buildings Using the 3D Model Generated from 2-Dimensional Cadastral Data
Having a 3-Dimensional model of high-rise buildings can be used in disaster management such as fire cases to reduce casualties. The fundamental dilemma in 3D building modeling is the unavailability of suitable data sources. However, available cadastral 2D maps could be used as low-cost and attainable resources for 3D building modeling. Smoke will be a great threat to people's health during a f...
متن کامل